Lunaphore COMET™ Spatial Proteomics

‌Highly multiplexed immunofluorescence

Propath's Lunaphore COMET - Stain, image, elute

‌Accelerate your research with spatial biology

‌As highly-multiplexed tissue imaging continues to demonstrate its potential for drug development, Propath now offers a comprehensive service for ultra-high plex immunofluorescence.

Image the location and abundance of multiple proteins of interest from a single tissue section
Gain large amounts of contextual information
Delve deeper into complex cell interactions
Wide range of applications in immuno-oncology, neuroscience and infectious diseases

‌Introducing the Lunaphore COMET^TM: robust and repeatable mIF staining

‌The COMET^TM is a high-throughput automated immunofluorescence staining platform, enabling imaging of up to 40 protein targets from a single tissue section.

‍The platform allows multiplexing with standard antibody libraries, with no conjugated or barcoding needed.

Using a sequential IF (seqIF^TM) approach means that highly multiplexed panels can be developed in days – not months – with high tissue preservation and signal stability.

‌Features of the COMET^TM mIF staining platform

As the only high-throughput solution available currently for hyperplex immunofluorescence staining, the COMET^TM offers several advantages:

Rapid staining
‌Fast exchange of reagents at the surface of the tissue results in short assay times
‌Unmatched uniformity
Continuous motion of fluids over the sample, enables superior signal uniformity with no gradient
‌Highly reproducible
Robust system and the automated protocols offer a high degree of consistency in results

‌High tissue preservation
10x less exposure of tissues to harsh chemicals and conditions preserves morphology and epitope stability
‌Quantifiable immunofluorescence
Signal intensity linearly correlates with biomarker expression, at cellular and subcellular levels
‌High throughput
10 plex: 24 slides/week
20 plex: 20 slides/week

High-plex immunofluorescence in practice: representative images produced at Propath

‌Conventional challenges of spatial biology: complexity and time

‌Lengthy optimization

‍Up to several months of optimization for a single assay

‌Manual and error-prone

‍Low reproducibility and high hands-on time

‌Complex reagents

‍Barcoding technologies, with upstream preparation, provider-dependent

‍‌Turnaround time

‍Workflows require several days to weeks to provide results

‌Propath’s solution for spatial biology using the Lunaphore COMET^TM

‌Rapid panel validation

‍Develop panels in days not months, with the ability to add-in markers to existing panels

‌End-to-end automation

‍Automated staining leads ensures high repeatability and reproducibility

‌Standard off-the-shelf reagents

‍Use reagents that you know

‌Rapid turnaround

High throughput enables quicker decision making

‌Lunaphore’s game-changing chip technology

‌Microfluidic chip enables staining and imaging
Pressure-driven system allowing for fast and uniform delivery of reagents on a tissue section within a closed chamber
Precisely controlled immuno-reaction within an extremely short incubation time enabled by Lunaphore’s FFeX^TM Technology

‌Sequential Immunoflurescence (seqIF^TM)

‌A validated core 10-plex immuno-oncology panel available from Propath

‌The panel can be modified with additional markers easily added-in
‌ Total protocol time: approx 6 hours

Marker	Subcellular Localisation	Cell lineage	Cell type
CD68	Cytosolic	Myeloid	Macrophage
FoxP3	Nuclear	Lymphoid	Regulatory T cell
CD8	Membrane	Lymphoid	T cell
PD1	Membrane	Lymphoid, Myeloid	Active T cell
PD-L1	Membrane	Lymphoid, Myeloid Cancer, Epithelial	Checkpoint
Ki67	Nuclear	All proliferating cells	Proliferative cells
CK	Membrane	Epithelial	Epithelium
CD4	Membrane	Lymphoid, Myeloid	T cell
CD20	Membrane	Lymphoid	B cell
CD3	Membrane	Lymphoid	T cell

‌Core 10-plex on breast cancer

‌CD20 CD68 PDL1 panCK CD3

‌FoxP3 PD1 CD8 Ki67 CD4

‌Core 10-plex on lung adenocarcinoma

‌CD20 CD68 PDL1 panCK CD3

‌FoxP3 PD1 CD8 Ki67 CD4

‌Core 10-plex on HNSCC

‌CD20 CD68 PDL1 panCK CD3

‌FoxP3 PD1 CD8 Ki67 CD4

FAQs

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Lunaphore COMET™ Spatial Proteomics

‌Highly multiplexed immunofluorescence

‌Accelerate your research with spatial biology

‌Introducing the Lunaphore COMETTM: robust and repeatable mIF staining

‌Features of the COMETTM mIF staining platform

High-plex immunofluorescence in practice: representative images produced at Propath

‌Conventional challenges of spatial biology: complexity and time

‌Propath’s solution for spatial biology using the Lunaphore COMETTM

‌Lunaphore’s game-changing chip technology

‌Sequential Immunoflurescence (seqIFTM)

‌A validated core 10-plex immuno-oncology panel available from Propath

‌Core 10-plex on breast cancer

‌Core 10-plex on lung adenocarcinoma

‌Core 10-plex on HNSCC

FAQs

Outsourced R&D - Done better.

‌Introducing the Lunaphore COMET^TM: robust and repeatable mIF staining

‌Features of the COMET^TM mIF staining platform

‌Propath’s solution for spatial biology using the Lunaphore COMET^TM

‌Sequential Immunoflurescence (seqIF^TM)