Lunaphore COMET Spatial Proteomics

‌Highly multiplexed immunofluorescence

Accelerate your research with spatial biology

‌As highly-multiplexed tissue imaging continues to demonstrate its potential for drug development, Propath now offers a comprehensive service for ultra-high plex immunofluorescence.

  • Image the location and abundance of multiple proteins of interest from a single tissue section
  • Gain large amounts of contextual information
  • Delve deeper into complex cell interactions
  • Wide range of applications in immuno-oncology, neuroscience and infectious diseases

Introducing the Lunaphore COMETTM: robust and repeatable mIF staining

The COMETTM is a high-throughput automated immunofluorescence staining platform,  enabling imaging of up to 40 protein targets from a single tissue section.

The platform allows multiplexing with standard antibody libraries, with no conjugated or barcoding needed.

Using a sequential IF (seqIFTM) approach means that highly multiplexed panels can be developed in days – not months –  with high tissue preservation and signal stability.

‌Features of the COMETTM mIF staining platform

As the only high-throughput solution available currently for hyperplex immunofluorescence staining, the COMETTM offers several advantages:

  • Rapid staining
    ‌Fast exchange of reagents at the surface of the tissue results in short assay times
  • ‌Unmatched uniformity
    Continuous motion of fluids over the sample, enables superior signal uniformity  with no gradient
  • ‌Highly reproducible
    Robust system and the automated protocols offer a high degree of consistency in results
  • ‌High tissue preservation
    10x less exposure of tissues to harsh chemicals and conditions preserves morphology and epitope stability
  • ‌Quantifiable immunofluorescence
    Signal intensity linearly correlates with biomarker expression, at cellular and subcellular levels
  • ‌High throughput
    10 plex: 24 slides/week
    20 plex: 20 slides/week

Conventional challenges of spatial biology: complexity and time

Lengthy optimization

Up to several months of optimization for a single assay
Manual and error-prone

Low reproducibility and high hands-on time
Complex reagents

Barcoding technologies, with upstream preparation, provider-dependent
Turnaround time

Workflows require several days to weeks to provide results

Propath’s solution for spatial biology using the Lunaphore COMETTM

Rapid panel validation

Develop panels in days not months, with the ability to add-in markers to existing panels
End-to-end automation

Automated staining leads ensures high repeatability and reproducibility
Standard off-the-shelf reagents

Use reagents that you know
‌Rapid turnaround

High throughput enables quicker decision making

Lunaphore’s game-changing chip technology

  • ‌Microfluidic chip enables staining and imaging
  • Pressure-driven system allowing for fast and uniform delivery of reagents on a tissue section within a closed chamber
  • Precisely controlled immuno-reaction within an extremely short incubation time enabled by Lunaphore’s FFeXTM Technology

‌Sequential Immunoflurescence (seqIFTM)

A validated core 10-plex immuno-oncology panel available from Propath

  • ‌The panel can be modified with additional markers easily added-in
  • ‌ Total protocol time: approx 6 hours

CD68
FoxP3
CD8
PD1
PD-L1
Ki67
CK
CD4
CD20
CD3
Cytosolic
Nuclear
Membrane
Membrane
Membrane
Nuclear
Membrane
Membrane
Membrane
Membrane
Myeloid
Lymphoid
Lymphoid
Lymphoid, Myeloid
Lymphoid, Myeloid
Cancer, Epithelial
All proliferating cells
Epithelial
Lymphoid, Myeloid
Lymphoid
Lymphoid
Myeloid
Lymphoid
Lymphoid
Lymphoid, Myeloid
Lymphoid, Myeloid
Cancer, Epithelial
All proliferating cells
Epithelial
Lymphoid, Myeloid
Lymphoid
Lymphoid
Marker Subcellular Localisation Cell lineage Cell type
CD68 Cytosolic Myeloid Macrophage
FoxP3 Nuclear Lymphoid Regulatory T cell
CD8 Membrane Lymphoid T cell
PD1 Membrane Lymphoid, Myeloid Active T cell
PD-L1 Membrane Lymphoid, Myeloid Cancer, Epithelial Checkpoint
Ki67 Nuclear All proliferating cells Proliferative cells
CK Membrane Epithelial Epithelium
CD4 Membrane Lymphoid, Myeloid T cell
CD20 Membrane Lymphoid B cell
CD3 Membrane Lymphoid T cell

‌Core 10-plex on breast cancer

‌CD20 CD68 PDL1 panCK CD3
‌FoxP3 PD1 CD8 Ki67 CD4

‌Core 10-plex on lung adenocarcinoma

‌CD20 CD68 PDL1 panCK CD3
‌FoxP3 PD1 CD8 Ki67 CD4

‌Core 10-plex on HNSCC

‌CD20 CD68 PDL1 panCK CD3
‌FoxP3 PD1 CD8 Ki67 CD4

FAQs

Outsourced R&D - Done better.

At Propath we approach biopharma R&D differently. Say goodbye to Large CRO frustrations –and hello to a more responsive, agile and efficient level of service.