Akoya Phenocycler and Phenoimager

‌Deep ultra high-plex, single-cell spatial phenotyping

‌Spatial phenotyping is transforming discovery research

‌Spatial phenotyping is driving a new wave of biologic discovery. Here’s how.

1. Cells don't live alone

Spatial phenotyping moves beyond simply knowing what cells are present in a sample to characterising how they cluster, which cells are adjacent, and their functional orientation. The different phenotypes associated with different cellular niches are highly predictive of tissue behaviour.

Cells don't live alone
Squeezing millions of insights out of each sample

2. Squeezing millions of insights out of each sample

‌Comprehensive spatial phenotyping can reveal cell diversity, co-expression patterns, cellular interactions, broader tissue architecture, and much more – all from a single FFPE tissue section.

3. Getting to know your neighbourhood

‌“Cellular neighbourhoods” is how a growing number of researchers are characterising the organization of diverse cell types across the tumour landscape.

Spatial phenotyping gives a deeper view into these different neighbourhoods and the way different cells influence their neighbours to reveal new insights into tumour pathology and immune response.

Getting to know your neighbourhood

‌Introducing the Phenocycler and PhenoImager platforms

‌A breakthrough solution for comprehensive and unbiased spatial phenotyping


Map 1 million cells in as little as 10 minutes


Image whole slides rapidly and ensure no cell is left behind


Single-cell and subcellular resolution


Analyse 100+ biomarkers from any sample type for deep spatial phenotyping

High throughput

Analyse 5 to 100+ slides per week


Analyze RNA and protein markers from the same samples

A complete solution for spatial phenotyping available at Propath

From Discovery to Validation to Clinical – the Akoya PhenoCycler and PhenoImager systems are part of an integrated spatial biology workflow from staining to analysis.

For Biomarker Discovery

Large high-plex panels

  • ‌Uses oligo-tagged mIF
  • ‌100+ plex protein targets
  • ‌10’s to 1,000 RNA transcripts
  • Single-cell and sub-cellular resolution
  • ‌8-30 samples / week
  • ‌Compatible with FFPE samples

For Translational Research

Targeted panels for high-throughput research

  • ‌Uses PhenoCycler Slides or Opal mIF
  • ‌6+ plex protein and/or RNA targets
  • Single-cell and sub-cellular resolution
  • ‌100+ samples / week
  • ‌Compatible with FFPE samples

‌Immune response in lung cancer FFPE tissue

‌PhenoImager multiplex immunofluorescence can reveal cellular phenotypes and interactions within the tumour microenvironment with whole slide spatial context and single-cell resolution.

Image shows a lung cancer FFPE tissue with little immune infiltration and high numbers of PD-1 and PD-L1 positive immune cells and FoxP3 positive cells in the surrounding microenvironment, indicating an ‘immune-excluded’ phenotype (DAPI– blue, CD8 – cyan, PD-1 – red, FoxP3 – yellow, CD-68 – orange, PD-L1 – green)

Credit: Akoya Biosciences

‌23-plex panel in psoriatic skin tissue

‌Image shows immune cell activity in psoriasis rash. Human fresh frozen tissue was stained with a 23-plex mixed epithelial and immune cell antibody panel to visualize epithelial and vascular markers and survey the immune landscape of skin inflammation in psoriasis.

(PanCK – white, CD49f – green, CD138 – red, Podoplanin – blue, CD45 – yellow, CD34 – cyan, CD31– magenta).

Credit: Akoya Biosciences

‌Propath’s integrated service for support across the biomarker development process

From Discovery to Validation to Clinical – the Akoya PhenoCycler and PhenoImager systems are part of an integrated spatial biology workflow from staining to analysis.

Assay Design

  • Support with assay design, development and validation
  • Access to pre-designed or custom antibody panels

Sample Prep, Staining and QC

  • Sample Procurement
  • Sample preparation, including H&E stains
  • Pathology Review
  • Tissue staining for multi-omic spatial analysis
  • Multiple platforms available in-house to support different research objectives

Image Acquisition

  • Multiple options for image acquisition, including the Akoya PhenoImager, Lunaphore COMET and 3D Histech platforms

Assay Design

  • Image Analysis Algorithm Development and Validation
  • Image Interpretation and Analysis
  • Reporting

Answer questions across the Research Continuum

Studies in human biology span a continuum from discovery to clinical and translational research. Different ends of this continuum require unique capabilities, warranting different workflows at different stages of research.

Need advice to get started?

‌Explore Propath’s Technology Access Programme for spatial proteomics and transcriptomics
‌Propath offers a range of flexible options to evaluate our different platforms for whole slide spatial tissue analysis.  We’re here to help and offer as much advice as needed to get you started.

‌Flexible options to meet your needs


Mouse or human

Tissue Format

Formalin-fixed, paraffin-embedded (FFPE), fresh frozen or TMAs

Antibody Panels

Ranging from validated, customized or pre-designed panels

Data Analysis

‌Comprehensive data analytics provided by Propath or QPTIFF files can be provided for direct analysis using a variety of third-party solutions.