Spatial phenotyping is driving a new wave of biologic discovery. Here’s how.
Spatial phenotyping moves beyond simply knowing what cells are present in a sample to characterising how they cluster, which cells are adjacent, and their functional orientation. The different phenotypes associated with different cellular niches are highly predictive of tissue behaviour.
Comprehensive spatial phenotyping can reveal cell diversity, co-expression patterns, cellular interactions, broader tissue architecture, and much more – all from a single FFPE tissue section.
“Cellular neighbourhoods” is how a growing number of researchers are characterising the organization of diverse cell types across the tumour landscape.
Spatial phenotyping gives a deeper view into these different neighbourhoods and the way different cells influence their neighbours to reveal new insights into tumour pathology and immune response.
A breakthrough solution for comprehensive and unbiased spatial phenotyping
From Discovery to Validation to Clinical – the Akoya PhenoCycler and PhenoImager systems are part of an integrated spatial biology workflow from staining to analysis.
PhenoImager multiplex immunofluorescence can reveal cellular phenotypes and interactions within the tumour microenvironment with whole slide spatial context and single-cell resolution.
Image shows a lung cancer FFPE tissue with little immune infiltration and high numbers of PD-1 and PD-L1 positive immune cells and FoxP3 positive cells in the surrounding microenvironment, indicating an ‘immune-excluded’ phenotype (DAPI– blue, CD8 – cyan, PD-1 – red, FoxP3 – yellow, CD-68 – orange, PD-L1 – green)
Credit: Akoya Biosciences
Image shows immune cell activity in psoriasis rash. Human fresh frozen tissue was stained with a 23-plex mixed epithelial and immune cell antibody panel to visualize epithelial and vascular markers and survey the immune landscape of skin inflammation in psoriasis.
(PanCK – white, CD49f – green, CD138 – red, Podoplanin – blue, CD45 – yellow, CD34 – cyan, CD31– magenta).
Credit: Akoya Biosciences
From Discovery to Validation to Clinical – the Akoya PhenoCycler and PhenoImager systems are part of an integrated spatial biology workflow from staining to analysis.
Studies in human biology span a continuum from discovery to clinical and translational research. Different ends of this continuum require unique capabilities, warranting different workflows at different stages of research.
