Propath offers a complete, turnkey service for GLP and GCP-compliant IHC staining, using automated and manual methods.
The team at Propath has substantial experience of developing and validating robust methods for experimental and regulated studies.
To generate meaningful study data that can be relied upon, we appreciate the importance of producing staining results that are consistent, sensitive and reproducible.
IHC is a complex, multi-parameter staining technique applied across variable fresh frozen and fixed paraffin embedded tissues. As a result, developing an optimum staining protocol is not always straightforward.
Our experience informs our view that high quality IHC with strong signal intensity and low background staining can only be achieved by paying careful attention to the following factors:
Whether developing an IHC method for a proprietary antibody clone or staining specimens from a large scale clinical trial; Propath has the experience and technology platforms to ensure your study is as successful as possible.
For routine IHC, we use the Roche Ventana Discovery Ultra and Leica Bond Rx III– both platforms well recognised for providing high quality, consistent and reproducible staining outcomes.
For more advanced multiplexed immunofluorescence, we offer access to the powerful Lunaphore Comet and Akoya PhenoCycler-Fusion and Pheno-imager platforms.
All IHC is conducted onsite by experienced scientists within our fully GLP and GCP compliant environment, providing Propath clients further confidence in our standards of quality assurance.
The team at Propath offer significant experience in developing and validating IHC and ISH assays for clinical, bio distribution, tissue cross-reactivity, efficacy, pharmacodynamics and toxicity studies.
We have worked with a broad range of biomarkers and antibody structures, and developed a range of strategies to overcome common technical challenges.