Molecular Pathology Services

GLP/GCP compliant IHC

Consistent and reproducible IHC

‌Propath offers a complete, turnkey service for GLP and GCP-compliant IHC staining, using automated and manual methods.

The team at Propath has substantial experience of developing and validating robust methods for experimental and regulated studies.

To generate meaningful study data that can be relied upon, we appreciate the importance of producing staining results that are consistent, sensitive and reproducible.

Propath’s key success factors for consistent, high quality IHC

‌IHC is a complex, multi-parameter staining technique applied across variable fresh frozen and fixed paraffin embedded tissues.  As a result, developing an optimum staining protocol is not always straightforward.

Our experience informs our view that high quality IHC with strong signal intensity and low background staining can only be achieved by paying careful attention to the following factors:

01
Thorough and thoughtful experimental design at the outset, with appropriate literature seaches
02
Careful tissue handling and optimum fixation – always avoiding over fixation
03
Use of high quality reagents and materials, paying particular attention to storage requirements
04
Thoughtful selection of antibody detection strategy
05
Precise control of the entire staining method, including heat induced antigen retrieval, primary and secondary antibody titre and incubation times
06
Use of appropriate positive and negative controls in all staining runs
07
A meticulous approach to troubleshooting when challenges arise
08
Confirmation of a robust method before moving to experimental samples

A range of staining platforms, each suited to different study objectives

‌Whether developing an IHC method for a proprietary antibody clone or staining specimens from a large scale clinical trial; Propath has the experience and technology platforms to ensure your study is as successful as possible.

For routine IHC, we use the Roche Ventana Discovery Ultra and Leica Bond Rx III– both platforms well recognised for providing high quality, consistent and reproducible staining outcomes.

For more advanced multiplexed immunofluorescence, we offer access to the powerful Lunaphore Comet and Akoya PhenoCycler-Fusion and Pheno-imager platforms.

All IHC is conducted onsite by experienced scientists within our fully GLP and GCP compliant environment, providing Propath clients further confidence in our standards of quality assurance.

Propath: An experienced provider of IHC staining services

‌The team at Propath offer significant experience in developing and validating IHC and ISH assays for clinical, bio distribution, tissue cross-reactivity, efficacy, pharmacodynamics and toxicity studies.

We have worked with a broad range of biomarkers and antibody structures, and developed a range of strategies to overcome common technical challenges.

  • ‌Automated and manual staining
    We offer automated staining on the Roche Ventana and Leica Bond platforms, providing consistent and reproducible outcomes
  • ‌Highly-multiplexed immunofluorescence
    We offer mIF with our Comet Lunaphore platform, currently offering up to 20-plex per slide, with 40-plex coming soon
  • ‌Pre-validated methods
    We have an extensive range of previously optimised methods for clinical and non-clinical tissue available for our clients
  • ‌Robust QA
    All processes at Propath are underpinned by well developed QA, data integrity and training protocols
  • ‌Multiplexed chromogenic IHC
    We are experienced at developing multiplexed methods, with up to four markers on a slide
  • ‌Rapid method development
    We are able to develop de novo methods for commercially available antibodies in as little as two weeks
  • ‌Method transfer validations
    We are able to transfer and validate methods developed by sponsors to our lab and platform
  • ‌100% traceability of samples
    We appreciate the irreplaceable nature of many tissues and therefore ensure complete traceability of all tissue from receipt to archive

A selection of our work

FAQs

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