Nanostring CosMX – SINGLE CELL MOLECULAR IMAGING

‌Deep, single-cell spatial phenotyping

‌Spatial Molecular Imaging

‌See more spatial biology from precious samples

Breaking new ground in spatial imaging, the NanoString CosMx provides up to whole-transcriptome in-situ analysis at cellular and subcellular resolution in FFPE and fresh frozen tissue.

‌Investigate morphologically-intact tissues at unprecedented plex, resolution and cell numbers
‌Explore the cell atlas, cell-cell interactions and phenotypes of the tissue microenvironment
Cloud-based data analytics, accelerating data-driven predictions and decisions

Imagery of slide data from Nanostring CosMx

A new standard for single-cell in situ analysis

High Resolution

S‌ingle molecule resolution for single cell and sub cellular analysis

Highest commercially available plex

‌Up to whole transcriptome and 100+ validated protein analytes

Multiomic

Analyse RNA and protein targets on the same slide
‍

The CosMx – At a glance

‌Works with real world samples
Compatible with FFPE and fresh frozen tissue
‌Cloud-based AI-powered analytics
Powerful bioinformatic pipeline driving deeper and faster data analysis

I‌maging of the complete tissue section
‌Flexibility to determine the imaging area and number of Fields of View (FOVs)
‌Interactive data visualisation
‌Enabling image sharing, collaboration and data visualisation

How the CosMx works

The CosMx uses automated fluidics and imaging to hybridise and visualise 16 sets of four fluoresecent reporters, enabling analysis of up to the whole transcriptome from a single 5μM section

‌How researchers are using the CosMx

‌Cell atlas and characterisation
Defining cell types, cell states, tissue microenvironment phenotypes, and gene expression networks within spatial context
Cell-cell interaction
Understanding biological process controlled by ligand-receptor interactions
‌Treatment effects
‌Quantifying changes in gene expression before and after treatment
‌Spatial biomarkers
‌Identifying single-cell subcellular biomarkers with spatial context

Advanced platforms for spatial biology available today at Propath

‌Our experience across a range of platforms for spatial biology allows us to recommend the most appropriate platform for each research project.

We understand the strengths and limitations of each platform – and how to get the best out of them.

COMET

Highly multiplexed sequential immunofluorescence for up to 40 protein on a single slide

‌Validated for GCP studies

nCounter, GeoMx and Cosmx

Integrated spatial multi-omics including up to whole transcriptome profiling from tissue to sub-cellular resolution

‌Validated for GCP studies

RNAScope and BaseScope

Robust in situ hybridization technology for rapid quantification of any RNA target in any tissue with spatial context

‌Validated for GCP studies

RNAscope and COMET

High-plex and focussed multi-omic mIF panels supporting discovery and translational studies

‌Validated for GCP studies

PhenoCycler and PhenoImager

High-plex and focussed mIF panels supporting discovery and translational studies

‌Validated for GCP studies

Xenium

Targeted, scalable spatial transcriptomics and multi-omics panels at subcellular resolution

‌Validated for GCP studies

nCounter, GeoMx and Cosmx

Integrated spatial multi-omics including up to whole transcriptome profiling from tissue to sub-cellular resolution

‌Validated for GCP studies

Proximity Ligation Assays

Automated PLA assays for the detection of protein-protein interactions and post- translational modifications

‌Validated for GCP studies

PhenoCycler and PhenoImager

High plex single-cell spatial phenotyping; and focused mIF panels for high-throughput translational research

‌Validated for GCP studies

‌Propath’s complete service for CosMx studies

We take care of the complete process from sample preparation to data analysis, with robust QA procedures and complete traceability of your precious samples.

01 .

Tell us about your study

We’ll explain the complete process and provide as much support as needed to help you make key decisions and generate meaningful data that achieves your objectives.

02 .

Select your panel and number of Fields of View (FOVs)

Choose from one of NanoString’s validated protein or RNA panels, with the option to spike-in additional protein targets if required.

03 .

Sample prep and hybridisation

We prepare your FFPE or fresh frozen samples ready for flow cell analysis, including hybridization of RNA specific probes and antibodies; and assembly of the CosMx flow cell.

04 .

CosMx molecular imaging

Our experienced staff run CosMx analysis within our dedicated NanoString lab based in the UK.

05 .

Communication and QA

Expect regular updates and as many collaborative discussions throughout your study as required. All our processes are supported by SOPs, traning and QA oversight, developed and refined over 30 years.

06 .

AI powered data analysis

We offer a comprehensive, service for data analysis and visualisations, powered by AI, hosted in the cloud and brilliant for collaboration across teams!

‌CosMx 64-plex Human Immuno-Oncology Protein Panel

Human I-O 64 plex panel	4-1BB	CD127	CD27	CD45	FABP4	ICAM1	LAG3	SMA
	B7-H3	CD138	CD3	CD45RA	Fibronectin	ICOS	LAMP1	STING
	Bcl-2	CD14	CD31	CD56	FOXP3	IDO1	NF-kB p65	TCF7
	Beta-catenin	CD15	CD34	CD68	GITR	IgD	p53	Tim-3
	CCR7	CD16	CD38	CD8	GZMA	IL-18	pan-RAS	Vimentin
	CD11b	CD163	CD39	CTLA4	GZMB	IL-1b	PD-1	VISTA
	CD11c	CD19	CD4	EGFR	Her2	iNOS	PD-L1	Rb IgG
	CD123	CD20	CD40	EpCAM	HLA-DR	Ki-67	PD-L2	Ms IgG1
Segmentation	panCK		Membrane Cocktail		Custom Selection		CD45

Nanostring CosMX – SINGLE CELL MOLECULAR IMAGING

‌Deep, single-cell spatial phenotyping

‌Spatial Molecular Imaging

‌See more spatial biology from precious samples

Reveal the full complexity of the tumour microenvironment

A new standard for single-cell in situ analysis

High Resolution

Highest commercially available plex

Multiomic

The CosMx – At a glance

How the CosMx works

‌How researchers are using the CosMx

Advanced platforms for spatial biology available today at Propath

‌Propath’s complete service for CosMx studies

01

.

Tell us about your study

02

.

Select your panel and number of Fields of View (FOVs)

03

.

Sample prep and hybridisation

04

.

CosMx molecular imaging

05

.

Communication and QA

06

.

AI powered data analysis

‌CosMx 64-plex Human Immuno-Oncology Protein Panel

Publications & Resources

David Rimm: paper on PD-1 checkpoint blockade

Propath’s brochure for NanoString services

FAQs