QUANTITATIVE Image Analysis

Multiplex Immunofluorescence Analysis

Advanced Image Analysis:
Qualitative images to
quantifiable data

As a spatial biology specialist, Propath’s bioinformatics service
enables collaboration at any stage of the data journey.

Whether you’re working with your own data or have generated
these with one of our other services, we’re equipped to deal with
your research needs. From image hosting through to advanced
analysis and statistical support, our expert team can accelerate
discovery through every pixel.

Image Analysis Service Menu

Basic, advanced, or bespoke packages
Different tier structure to suit your needs
Image QC
Quantitative and qualitative image QC metrics
Tissue segmentation
Understanding cellular relationships within separate tissue
categories
Cell segmentation & phenotyping
Defining cellular boundaries using nuclei dilation or bespoke
AI-algorithms
Advanced Data Analysis
Downstream data analysis to aid interpretation

Introducing our service

Image hosting
Cloud-hosted image management with PathCore, featuring advanced annotation tools and seamless team access and collaboration within a web browser
Fully Integrated Service
A single end-to-end solution for sample processing, staining. imaging, hosting, analysis, and reporting
Platform Agnostic
Our services can analyse images regardless of the originating platform. If you have images, we can ensure seamless integration and comprehensive analysis

GCP Image Analysis
We offer GCP-validated whole slide analysis using Visiopharm, leveraging deep learning and artificial intelligence for exploratory
endpoints
Standalone Service
Tailored, specific services to complement existing workflows, offering flexibility and cost-effectiveness
Multi-omics
Our services allow integration of multiple data sources, both spatial and non-spatial: IHC, H&E/Histology, mIF, IMC, spatial transcriptomics, and bulk sequencing

A closer look at image analysis tiers

Standard	Advanced	Bespoke
Routine tissue segmentation into 2 categories	Bespoke tissue segmentation into up to 3 categories	Get in touch
Cell segmentation	Addition of extra training to cell segmentation, if required
Cell phenotyping (up to X markers)	Cell phenotyping (up to X markers)
Density analysis, average counts within, nearest neighbour	Density analysis, average counts within, nearest neighbour, distance to category edge, neighbourhood analysis
Data provided as excel file	Data provided as excel file and high-resolution figures

GCP validated workflow

Step 1

Image to data

Step 2

Organise and label data

Step 3

Extract insights

Image Sharing & Hosting

01

High-resolution image display: view natively rendered whole slide images in the browser

02

Zoom and navigation controls: full zoom, pan and rotate controls for a natural viewing experience

03

Full display controls: select channel combinations, set colours and adjust histograms to highlight the biology

04

Annotation tools: Integrated tools enabling identification and markup of features and regions of interest

05

Integrated analysis workflow: Bidirectional interface with Visiopharm to ensure a seamless training and
review of feature detection algorithms

06

Cloud-hosted: easily share and distribute images and annotations with your team with a web link, or lock your
images with account-restricted access.

Advanced platforms for spatial biology available today at Propath

‌Our experience across a range of platforms for spatial biology allows us to recommend the most appropriate platform for each research project.

We understand the strengths and limitations of each platform – and how to get the best out of them.

‌Lunaphore COMET
‍

Highly multiplexed sequential immunofluorescence for up to 40 protein on a single slide

‌Validated for GCP studies

‌Akoya PhenoCycler and PhenoImager

‌Ultra-high plex single-cell spatial phenotyping; and focused mIF panels for high-throughput translational research

‌Validated for GCP studies

‌RNAScope and BaseScope
‍

Robust in situ hybridization technology for rapid quantification of any RNA target in any tissue with spatial context

Navinci Proximity Ligation
Assays

Automated PLA assays for the
detection of protein-protein
interactions and post-
translational modifications

Take a look at some of our work…

FAQs

A selection of our work

01

‌Human lymphoid tissue section showing six channels from a 10-plex immunofluorescence stain, produced on Propath’s Lunaphore Comet platform.

CD4 green, CD8 orange, PD-1 red, PD-L1 magenta, FOXP3 white, DAPI blue

02

‌The very same section of human lymphoid tissue as previous image but now showing another five channels of the same 10-plex immunofluorescence stain.

CD20 green, CD3 red, CK magenta, Ki67 white, CD68 orange

Lymph node slide showing five channels of the 10-plex immunofluorescence stain.

03

‌Non-small-cell lung carcinoma section with four colour fluorescent morphology stain.Image highlights epithelial tumour and immune infiltrate with region of interest and tissue segmentation overlays.

DNA, CD45, CD68, PanCK

04

‌Dual immunofluorescent staining of pSMAD (Cy5 fluorophore, orange) and a proprietary biomarker (FAM fluorophore, green) in pancreatic cancer tissue.

Strong positive pSMAD staining present in pancreatic islets, with some areas of co-localisation present indicated by bright yellow staining. DAPI nuclear stain is shown in blue.

05

‌GFAP (glial fibrillary acidic protein) staining of glial cells in the hippocampus.

Chromogenic DAB staining.

06

‌Chromogenic ISH staining of human NPY2 receptor in an animal model brain.

Only the human NPY2 receptor is specifically detected and not the endogenous NPY2 receptor.

07

‌Spatial protein and RNA analysis of a section of colorectal carcinoma using the NanoString GeoMx with IHC overlays.

Top left: GeoMx ROI selection for protein panel

Top right: GeoMx ROI selection for Whole Transcriptome RNA panel

Middle right: Dual IHC stain for CD4 in purple and CD8 in yellow

Bottom right: IHC stain for PD-LI

08

‌Jones’ methenamine silver stain in mini-pig kidney demonstrating basement membranes.

09

‌Masson’s Trichrome stain with aniline blue counterstain of kidney, demonstrating collogen in blue/dark blue; muscle red blood cells and fibrin in red; and nuclei in blue/black.

10

‌H&E stain of skin and mammary gland, showing epidermis, dermis, hair follicles, fat cells and mammary gland.

11

‌H&E stain of testis/epididymis demonstrating testis (seminiferous tubules containing spermatocytes), interstitial Leydig cells; and epididymis: initial segment (dense tubular area above head), head (caput), corpus(middle), caput (tail).

12

‌Verhoff’s Van Gieson stain of aorta demonstrating aorta, tunica adventitia, tunica media, tunica intima, with collagen in red and elastic fibres in black.

13

‌H&E stain of eye demonstrating cornea, iris, lens, retina, choroid, optic nerve and Hardarian gland.

Outsourced R&D - Done better.

At Propath we approach biopharma R&D differently. Say goodbye to Large CRO frustrations –and hello to a more responsive, agile and efficient level of service.

Schedule a call Get in touch

QUANTITATIVE Image Analysis

Multiplex Immunofluorescence Analysis​

Advanced Image Analysis:Qualitative images toquantifiable data​

Image Analysis Service Menu​

Introducing our service​​

A closer look at image analysis tiers​

GCP validated workflow​