Multiplex IHC staining
Multiplex IHC allows the simultaneous detection of multiple proteins of interest in a single tissue sample. Increasing availability of multiple biomarkers for molecular diagnosis and limited availability of rare patient biopsy material has led to the development of multiplexed assays.
Novel multiplex IHC methods that have been developed at Propath allow the identification of four to five discrete antigens in a single section, visualised by brightfield or fluorescent microscopy.
Multiplex IHC is particularly attractive in the field of immuno-oncology as it enables detection of spatial relationships and an understanding tumour microenvironment. Multiplex IHC followed by quantitative image analysis addresses the issue of tumour heterogeneity leading to better therapeutic regimens.
Custom developed image analysis algorithms developed by Propath and its partners enables quantification of immune cell numbers, immune cell co-localisation and spatial relationships within the tumour microenvironment. Quantification of such parameters is particularly beneficial in understanding the mechanism of action of immunotherapies.
IHC/ISH AND IHC/SPECIAL STAIN MULTIPLEX ASSAYS
Multiplex assays at Propath are not limited only to IHC biomarkers. The team at Propath has experience of developing IHC/ISH multiplex and IHC/Special Stain multiplex assays. These multiplex assays have proven to be valuable in establishing maximum study data from minimal tissue samples and have also been used in the development of companion diagnostic tests.
Images: Access to the latest chromogenic detection kits. Displaying some of the newly available chromogenic detection kits with Ki67 IHC in human tonsil tissue. Access to different chromogenic detection systems allows multiplexing and visualisation of multiple biomarkers in the same tissue section.