‌Nanstring nCounter RUO PAM50 Analysis

Rapid quantification of the PAM50 signature genes with data analysis

PAM50 breast cancer sample analysis

PAM50 is a list of 50 genes that classify breast cancer into one of four intrinsic subtypes: Luminal A, Luminal B, HER2-enriched, Basal-like and Normal-Like¹.

These subtypaes have been shown to be prognostic in both un-treated (i.e. no adjuvant systemic therapy) and tamoxifen treated patient populations.

Using NanoString’s RUO PAM50 panel, quantification of the PAM50 genes can now be performed in surgically resected breast cancer samples more rapidly and reproducibly than is possible using qPCR or RNASeq.

^1.The Cancer Genome Atlas. Comprehensive molecular portraits of human breast tumours. Nature. 2012;490(7418):61–70. doi: 10.1038/nature11412.

Propath: An experienced service provider for nCounter PAM50 Analysis

Propath offers a complete, managed service for analysis of breast cancer samples using NanoString’s PAM50 panel.

Analysis is for Research Use Only.

Simply send us your samples and receive publication ready data in as little as three weeks.

Request a Quote

Experienced and helpful staff
‌PhD-level scientists experienced at running the PAM50 assay and nCounter studies
IHC and H&E staining
Ability to produce and report on IHC and H&E stains from the same sample
‌Robust QA
All processes at Propath are underpinned by well developed QA, data integrity and training protocols

A complete service
From RNA extraction and QC analysis through to data analysis and reporting
Rapid turnaround
Studies can be run within as little as 3 weeks, including production of a PAM50 Single Signature Data Analysis Report
‌100% traceability of samples
We appreciate the irreplaceable nature of many tissues and therefore ensure complete traceability of all tissue from receipt to archive

PAM50 RUO analysis using the nCounter – At a glance

Robust and reproducible
Uses direct detection of fluorescent molecular barcodes: digital precision, with reduced bias and increased reproducibility
The PAM50 CodeSet
Contains probes for all 50 genes within the PAM50 signature as well as 8 reference genes
Faster than qPCR
From samples to publication-ready data in as little as three weeks

Good for FFPE samples
And with difficult samples — even severely degraded RNA can be viable; with low sample input requirements
Customisable
Option to add up to 55 additional target genes with Panel-Plus
Data quality
Assay controls are included to ensure that test samples and the test process meet pre-defined quality thresholds

Propath’s workflow for RUO PAM50 Analysis

01 .

Your samples

We handle them as though they are precious - with 100% traceability through our facility

02 .

Quality of service

Professional and scientifically led, with responsive customer communication

03 .

Rapid turnaround

Say goodbye to long waits. We can provide publication-ready data in as little as three weeks

04 .

PAM50 data analysis

Our service includes a PAM50 data analysis report. If clinical tumour size is provided, the PAM50 report will also include the Risk of Recurrence (ROR) score

05 .

Integrated histopathology support for IHC and H&E

We offer H&E and IHC stains from the sample sample, allowing data interpretation in the context histological analysis

06 .

Robust QA

From management of your samples to our people, process and facilities a strong culture of quality assurance runs through everything we do

Advanced platforms for spatial biology available today at Propath

‌Our experience across a range of platforms for spatial biology allows us to recommend the most appropriate platform for each research project.

We understand the strengths and limitations of each platform – and how to get the best out of them.

‌NanoString GeoMx and CosMx

‌Whole slide spatial analysis of 18,000 RNA transcripts (GeoMx) and 1,000 RNA transcripts at sub cellular level (CosMx)

‌Validated for GCP studies

‌Lunaphore COMET
‍

Highly multiplexed sequential immunofluorescence for up to 40 protein on a single slide

‌Validated for GCP studies

‌Akoya PhenoCycler and PhenoImager

‌Ultra-high plex single-cell spatial phenotyping; and focused mIF panels for high-throughput translational research

‌Validated for GCP studies

‌RNAScope and BaseScope
‍

Robust in situ hybridization technology for rapid quantification of any RNA target in any tissue with spatial context

Outsourced R&D - Done better.

At Propath we approach biopharma R&D differently. Say goodbye to Large CRO frustrations –and hello to a more responsive, agile and efficient level of service.

Schedule a call Get in touch

‌Nanstring nCounter RUO PAM50 Analysis

Rapid quantification of the PAM50 signature genes with data analysis

PAM50 breast cancer sample analysis

Propath: An experienced service provider for nCounter PAM50 Analysis

PAM50 RUO analysis using the nCounter – At a glance

Propath’s workflow for RUO PAM50 Analysis

01

.

Your samples

02

.

Quality of service

03

.

Rapid turnaround

04

.

PAM50 data analysis

05

.

Integrated histopathology support for IHC and H&E

06

.

Robust QA

01

.

Tell us about your study

02

.

Select your panel

03

.

Send us your samples

04

.

Sample preparation

05

.

Hybridisation of RNA with molecular barcodes

06

.

Digital counting

07

.

Data analysis

08

.

QA review and audits

Advanced platforms for spatial biology available today at Propath

Outsourced R&D - Done better.