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GLP and GCP compliant histology

For over 30 years Propath has offered a full range of contract histology services – from routine to more complex techniques, across all species.  Services include tissue trimming, embedding, sectioning, H&E staining and special stains, all provided by highly trained and experienced technicians within a fully GLP and GCP compliant environment. 

We operate an integrated quality management system throughout our facility covering all aspects of our service, including handling of tissues, subject confidentiality, study documentation, Quality Assurance, staff training, equipment maintenance and use of validated SOPs. 

Our Study Directors and Principal Investigators have many years of conducting multi-site GLP studies and are familiar with all required regulatory documentation, including phase plans and study protocols. 

Propath is experienced with all species and permitted to work with human tissue specimens under license from the UK Human Tissue Authority.


A SMALL SELECTION OF SPECIAL STAINS
 

01 Masson’s Trichrome stain demonstrating collagen in blue/green, muscle, red blood cells and fibrin in red nuclei in blue/black.   

01 Masson’s Trichrome stain demonstrating collagen in blue/green, muscle, red blood cells and fibrin in red nuclei in blue/black. 

 

02 Picro Sirius Red special stain – collagen fibres are stained red on a pale-yellow background with nuclei staining brown/black..   

02 Picro Sirius Red special stain – collagen fibres are stained red on a pale-yellow background with nuclei staining brown/black.. 

 

03 Mucicarmine special staining on mini-pig colon providing yellow connective tissue with pink/light red mucin producing cells  

03 Mucicarmine special staining on mini-pig colon providing yellow connective tissue with pink/light red mucin producing cells

 

04 Jones’ methenamine silver stain in mini-pig kidney demonstrating basement membranes    

04 Jones’ methenamine silver stain in mini-pig kidney demonstrating basement membranes

 

 

05 Verhoeff’s Van Gieson on aorta section demomstrating elastic fibres in black, collagen in red  

05 Verhoeff’s Van Gieson on aorta section demomstrating elastic fibres in black, collagen in red

 

06 Hall’s bilirubin stain on section of human liver demonstrating bile pigment in green (olive-emerald), muscle and cytoplasm in yellow and collagen in red  

06 Hall’s bilirubin stain on section of human liver demonstrating bile pigment in green (olive-emerald), muscle and cytoplasm in yellow and collagen in red

 

All images produced and copyright Propath UK Limited, 2017


The Propath standard 

Producing high quality, precise, consistent slides – in high throughput – is no small challenge.  We go to great lengths to achieve this by focusing relentlessly on every small detail at every discrete stage of the histology process.   

This includes, for example, controlling:

01
The quality and origin of solvents used in processing to ensure good tissue preparation
02
The precise temperature of our water baths to enable complete flattening of tissues
03
The quality of microtome blades to produce clean, sharp sections
04
The composition of water used in our proprietary staining method to create exceptional stains
05
The concentration of decal fluids to ensure gentle and effective demineralisation of bones
06
The level of contaminants within our work environment to minimise artifact on slides
07
Careful packaging of slides to prevent damage during transit
08
And having a low threshold for rejection of slides that are not up to the Propath standard.

Please contact us if you would like to receive samples of our slides.


Summary of Propath's research histology services

 

 
01
Tissue preparation according to standard or client specific trimming planes
02
Expertise in inhalation studies including precise trimming of nasal cavities using Exakt cutting system
03
Processing of large bones and joints – including femur and tibia joints
04
Precision sectioning of FFPE and frozen tissue blocks
05
100% quality control prior to dispatch
06
Customised blocking schemes
07
Megablock soft and hard decalcified tissues
08
Standard H&E staining and special stains
09
Step serial sections to identify specific tissue structures, for example in brain or larynx specimens
10
Use of modified decalcifaction fluid for bonesto preserve tissue integrity and epithelial surfaces
11
Paraffin wax embedding to standard orspecific size and orientation requirements
12
Specific cassette and slide printing according to sponsor requirements
13
Use of Propath or client SOPs
14
Archive ready packaging of residual tissues
15
International transportation and documentation
16
Door-to-door collection and delivery

We would be delighted to discuss your specific histology requirements – please do contact us.

 

SPECIAL histology stains available at Propath

Propath offers a wide range of validated empirical staining methods, including but not limited to:

Alcian blue PAS
Hall’s Bilirubin stain
Jones Methenamine silver
Masson’s Trichrome
MSB
Mucicarmine
Oil Red O  

 

PAS  
PASD
PBR
Perls’ Prussian Blue
Picro Sirius Red
PTAH
Verhoeff’s Van Gieson 


GLP histology studies 

Propath has many years of experience of conducting the histology phase of multi-site GLP studies.   For such studies the company is able to offer its own experienced Principle Investigators to ensure GLP compliance for the phase performed at Propath.  A phase report can be provided and a study-specific QA audit performed, as required.   A statement of GLP compliance is provided for all studies.   Sponsors are always welcome to visit Propath and conduct their own onsite QA assessment. 

Related services available from Propath

  • Consultancy and advice on study design, regulatory issues and GLP documentation
  • Expert histopathology evaluation and reporting
  • High-resolution digital scanning of slides
  • Automated IHC and ISH using the Roche Ventana Discovery XT platform
  • Organisation and collaboration of peer reviews